High-throughput process development: Chromatography media volume definition

Prediction of chromatographic separations in liter scale with the help of data gathered using a microliter format is challenging, as both scale effects and differences in the formats used (plates vs. packed minicolumns) need to be addressed. The use of microplates in high‐throughput process development (HTPD) studies has become a routine. While this method is typically used for investigating chromatography conditions such as salt and pH, it can...