RNAi Knockdown of a Salivary Transcript Leading to Lethality in the Pea Aphid,Acyrthosiphon pisum

Injection of siRNA (small interfering RNA) into parthenogenetic adult pea aphids (Acyrthosiphon pisum) is shown here to lead to depletion of a target salivary gland transcript. The siRNA was generated from double stranded RNA that covered most of the open reading frame of the transcript, which we have called C002. The C002 transcript level decreases dramatically over a 3-day period after injection of siRNA. With a lag of 1 to 2 days, the...