SiRNA Transfection with Cholesterol Modification from Electrospun PET Facilitates Gene Silencing in Human Aortic Smooth Muscle Cells

Anastomotic intimal hyperplasia (AIH) remains the leading cause for prosthetic arterial graft failure. RNAi is a promising tool to silence genes such as Thrombospondin-2 (TSP-2), previously shown by our group to be upregulated in AIH. The present study is aimed at delivering modified TSP-2 siRNA (3’cholesterol tagged TSP-2 siRNA) from electrospun polyethylene terephthalate (ePET) and therefore knocking down TSP-2 gene and ultimately inhibiting...