Loss of glutamate dehydrogenase in Ralstonia solanacearum alters dehydrogenase activity, extracellular polysaccharide production and bacterial virulence

Metabolism in Ralstonia solanacearum, which causes lethal wilt on Solanaceous plants, is poorly understood. In this study, we selected a Tn5-inserted mutant of R. solanacearum SL341 showing various phenotypic changes and altered virulence. When the gdhA gene encoding NAD(P)+-dependent glutamate dehydrogenase was disrupted, the gdhA mutant of SL341 (SL341P2) was defective in red colony development on tetrazolium chloride-amended medium and showed...