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Propofol attenuates angiotensin II-induced apoptosis in human coronary artery endothelial cells

Published on Oct 1, 2011in BJA: British Journal of Anaesthesia6.199
· DOI :10.1093/bja/aer197
Jiawei Chen11
Estimated H-index: 11
(Fudan University),
Wei Chen3
Estimated H-index: 3
(Fudan University)
+ 3 AuthorsZhiming Tan13
Estimated H-index: 13
(Fudan University)
Abstract
Background Angiotensin II (Ang II) induces oxidative stress and apoptosis in vascular endothelial cells. We hypothesized that propofol may attenuate Ang II-induced apoptosis in human coronary artery endothelial cells (HCAECs) and aimed to identify the underlying mechanisms. Methods Endothelial cells were pre-treated with propofol and then stimulated with Ang II. Apoptosis was examined by TUNEL, DNA laddering, and caspase-3 activity assays. The effect of propofol on Ang II-modulated NADPH oxidase expression and activity, nitric oxide synthase III (NOSIII) expression and phosphorylation and activity, lipid peroxidation, superoxide anion generation, nitric oxide production, caspase activity, and protein expression of cytochrome c , Bcl-2, and C-IAP-1 were measured. Results Ang II induced apoptosis, which was attenuated by 50 µM propofol ( P P P P P c release, and the expression of Bcl-2 and C-IAP-1. In addition, propofol inhibited Ang II-induced caspase-9 ( P P Conclusions Propofol protected HCAECs from Ang II-induced apoptosis by interfering with the generation of oxidative stress and redox-sensitive apoptotic pathways.
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