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High Diversity of Ligularia dictyoneura in Chemical Composition and DNA Sequence

Published on Dec 1, 2007in Chemistry & Biodiversity1.449
· DOI :10.1002/cbdv.200790237
Hajime Nagano17
Estimated H-index: 17
(Ochanomizu University),
Yukiko Iwazaki3
Estimated H-index: 3
(Ochanomizu University)
+ 6 AuthorsRyo Hanai23
Estimated H-index: 23
(Rikkyo University)
Abstract
The chemical composition of root extracts of the title species collected at 20 different places in the Hengduan Mountains of China was examined. From these samples, a total of 17 eremophilane derivatives were isolated, three of which were new franoeremophilane derivatives: 3 beta-acetoxy-6 beta-(angeloyloxy)furanoeremophilan-10 beta-ol, 1 alpha-acetoxyfuranoeremophilan-15,6 alpha-olide, and 6 beta-[2-(hydroxymethyl)prop-2-e noyloxy] furanoeremophil-1(10)-ene. Based on the chemical composition, the samples could be classified into as many as seven types: one type containing non-furanoeremophilane derivatives and the other six containing furanoeremophilane derivatives with different oxidation levels. Results of DNA sequencing of the atpB-rbcL region and the internal transcribed spacers of the ribosomal RNA gene also indicated a high diversity in the species.
  • References (45)
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References45
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#1Chiaki Kuroda (Rikkyo University)H-Index: 20
#2Kota Kiuchi (Rikkyo University)H-Index: 2
Last. Ryo Hanai (Rikkyo University)H-Index: 23
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ne chemical constituents of the root extracts and the nucleotide sequences of the atpB-rbcL intergenic region of Ligularia latihastata and L. villosa, collected in northwestern Yunnan Province, were studied. In the twelve collected samples of L. latihastata, two major benzofurans. 5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran (1) and euparin (2) were detected as major components. The minor compound (2R*,3S*)-5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl (2Z)-2[(acetoxy)me...
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The intraspecific diversity in L. tsangchanensis collected in the Chinese Provinces Yunnan and southwestern Sichuan was studied by chemical and genetic approaches. ne samples collected in Yunnan were found to contain cacalol (1) as the sole major component, while samples from Sichuan contained 7 alpha- and 7 beta-eremophila-9,11-dien-8-one (5 and 6) as well as the 3 alpha-angeloyloxy derivative 7 as major components. In addition, the sequences of the internal transcribed spacers (ITSs) of the ri...
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Extensive application of 1D and 2D NMR methodology, combined with molecular modeling, allowed the complete 'H and 13C NMR assignments of eremophilanolides from Senecio toluccanus. Comparison of the experimental 'H, 'H coupling constant values with those generated employing a generalized Karplus-type relationship, using dihedral angles extracted from MMX and DFT calculations, revealed that the epoxidized erernophilanolides 1 and 2 show conformational rigidity at room temperature, whereas molecule...
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