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Insulin on hydrogen peroxide-induced oxidative stress involves ROS/Ca2+ and Akt/Bcl-2 signaling pathways

Published on Mar 1, 2014in Free Radical Research2.83
· DOI :10.3109/10715762.2013.869588
M. Ramalingam1
Estimated H-index: 1
(Kyung Hee University),
S.-J. Kim1
Estimated H-index: 1
(Kyung Hee University)
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Abstract
AbstractOxidative stress is induced by excess accumulation of reactive oxygen and nitrogen species (RONS). Astrocytes are metabolically active cells in the brain and understanding astrocytic responses to oxidative stress is essential to understand brain pathologies. In addition to direct oxidative stress, exogenous hydrogen peroxide (H2O2) can penetrate biological membranes and enhance formation of other RONS. The present study was carried out to examine the role of insulin in H2O2-induced oxidative stress in rat astrocytic cells. To measure changes in the viability of astrocytes at different concentrations of H2O2 for 3 h, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)-based assay was used and 500 μM H2O2 was selected to establish a model of H2O2-induced oxidative stress. Further assays showed that 3 h of 500 μM H2O2-induced significant changes in the levels of lactate dehydrogenase (LDH), reactive oxygen species (ROS) and calcium ion (Ca2+) in C6 cells, with insulin able to effect...
  • References (41)
  • Citations (19)
Cite
References41
Newest
Published on Jul 1, 2013in Biochimica et Biophysica Acta3.79
Benjamin Bonneau8
Estimated H-index: 8
(University of Lyon),
Julien Prudent14
Estimated H-index: 14
(University of Lyon)
+ 1 AuthorsGermain Gillet13
Estimated H-index: 13
(University of Lyon)
Abstract The existence of the bcl-2 (B-cell lymphoma-2) gene was reported nearly 30 years ago. Yet, Bcl-2 family group of proteins still surprises us with their structural and functional diversity. Since the discovery of the Bcl-2 family of proteins as one of the main apoptosis judges, the precise mechanism of their action remains a hot topic of intensive scientific research and debates. Although extensive work has been performed on the role of mitochondria in apoptosis, more and more studies po...
Published on Sep 1, 2012in Free Radical Research2.83
Rituraj Niranjan6
Estimated H-index: 6
(Central Drug Research Institute),
Chandishwar Nath33
Estimated H-index: 33
(Central Drug Research Institute),
Rakesh Shukla32
Estimated H-index: 32
(Central Drug Research Institute)
AbstractMelatonin has been known to affect a variety of astrocytes functions in many neurological disorders but its mechanism of action on neuroinflammatory cascade and alpha-7 nicotinic acetylcholine receptor (α7-nAChR) expression are still not properly understood. Present study demonstrated that treatment of C6 cells with melatonin for 24 hours significantly decreased lipopolysaccharide (LPS) induced nitrative and oxidative stress, expressions of cyclooxigenase-2 (COX-2), inducible nitric-oxid...
Published on Aug 1, 2012in Journal of Neural Transmission2.90
Mahesh Ramalingam6
Estimated H-index: 6
(Kyung Hee University),
Sung-Jin Kim8
Estimated H-index: 8
(Kyung Hee University)
The continuous production and efflux of reactive oxygen/nitrogen species from endogenous and exogenous sources can damage biological molecules and initiate a cascade of events. Mitochondria are pivotal in controlling cell survival and death. Cumulative oxidative stress, disrupted mitochondrial respiration, and mitochondrial damage are related with various neurodegenerative disorders, including Alzheimer’s disease, Parkinson’s disease, and others. Biochemical cascades of apoptosis are mediated in...
Published on Aug 1, 2012in Food and Chemical Toxicology3.77
Lingling Zhang2
Estimated H-index: 2
(Fourth Military Medical University),
Wenjun Ding1
Estimated H-index: 1
(Fourth Military Medical University)
+ 5 AuthorsJianzong Chen3
Estimated H-index: 3
(Fourth Military Medical University)
Abstract Oxidative stress plays an important role in the pathogenesis of Parkinson’s disease (PD). Salidroside (SAL), a phenylpropanoid glycoside isolated from Rhodiola rosea L ., can exert potent antioxidant properties. In this study, we investigated the protective effects, and the possible mechanism of action, of SAL against 1-methyl-4-phenylpyridinium (MPP + )-induced cell damage in rat adrenal pheochromocytoma PC12 cells. Pretreatment of PC12 cells with SAL significantly reduced the ability ...
Published on Feb 1, 2012in Journal of Molecular Neuroscience2.58
Xiao-Qing Tang7
Estimated H-index: 7
(University of South China),
Yuan-Yuan Zhuang7
Estimated H-index: 7
(University of South China)
+ 4 AuthorsBi Hu8
Estimated H-index: 8
(University of South China)
We previously reported that hydrogen sulfide (H2S) produces protection in PC12 cells during 1-methy-4-phenylpyridinium ion (MPP+) challenge. The present study aims to clarify the mechanisms underlying the neuroprotective effects of H2S. We showed that both glybenclamide, an ATP-sensitive potassium (KATP) channel blocker, and LY294002, a specific PI3K–AKT pathway inhibitor, reversed the neuroprotective effect of NaHS (a H2S donor) against MPP+-induced cytotoxicity to PC12 cells and that NaHS up-r...
Published on Oct 31, 2009in Biomolecules & Therapeutics3.09
Ramalingam Mahesh1
Estimated H-index: 1
,
Sung-Jin Kim1
Estimated H-index: 1
Abstract - Insulin appears to play a role in brain physiology, and disturbances of cerebral insulin signalling and glucose homeostasis are implicated in brain pathology. The objective of the present study was to investigate the protective effects of insulin under conditions of oxidative stress induced by hydrogen peroxide (H 2 O 2 ) in C6 glial cells. Insulin at concentration of 10 −7 M could prevent 12 h H 2 O 2 -induced cell death. The formation of reactive oxygen species (ROS), nitric oxide (...
Published on May 1, 2009in Biochimie3.36
Mahdieh Sadidi3
Estimated H-index: 3
(UM: University of Michigan),
Stephen I. Lentz11
Estimated H-index: 11
(UM: University of Michigan),
Eva L. Feldman81
Estimated H-index: 81
(UM: University of Michigan)
Abstract Reactive oxygen species such as hydrogen peroxide (H 2 O 2 ) are involved in many cellular processes that positively and negatively regulate cell fate. H 2 O 2 , acting as an intracellular messenger, activates phosphatidylinositol-3 kinase (PI3K) and its downstream target Akt, and promotes cell survival. The aim of the current study was to understand the mechanism by which PI3K/Akt signaling promotes survival in SH-SY5Y neuroblastoma cells. We demonstrate that PI3K/Akt mediates phosphor...
Published on Dec 1, 2008in Neurochemistry International3.99
Laura E. Gonzalez2
Estimated H-index: 2
(Facultad de Ciencias Exactas y Naturales),
A. Ana Juknat1
Estimated H-index: 1
(Facultad de Ciencias Exactas y Naturales)
+ 2 AuthorsMónica L. Kotler15
Estimated H-index: 15
(Facultad de Ciencias Exactas y Naturales)
Manganese induces the central nervous system injury leading to manganism, by mechanisms not completely understood. Chronic exposure to manganese generates oxidative stress and induces the mitochondrial permeability transition. In the present study, we characterized apoptotic cell death mechanisms associated with manganese toxicity in rat cortical astrocytes and demonstrated that (i) Mn treatment targets the mitochondria and induces mitochondrial membrane depolarization followed by cytochrome c r...
Published on Jun 1, 2007in Cell36.22
Brendan D. Manning51
Estimated H-index: 51
(Harvard University),
Lewis C. Cantley169
Estimated H-index: 169
(BIDMC: Beth Israel Deaconess Medical Center)
The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central node in cell signaling downstream of growth factors, cytokines, and other cellular stimuli. Aberrant loss or gain of Akt activation underlies the pathophysiological properties of a variety of complex diseases, including type-2 diabetes and cancer. Here, we review the molecular properties of Akt and the approaches used to characterize its true cellular targets. In addition, we discuss those Akt substrates that are...
Published on Feb 1, 2007in Clinical Cancer Research8.91
John P. Fruehauf33
Estimated H-index: 33
,
Frank L. Meyskens57
Estimated H-index: 57
New insights into cancer cell–specific biological pathways are urgently needed to promote development of rationally targeted therapeutics. Reactive oxygen species (ROS) and their role in cancer cell response to growth factor signaling and hypoxia are emerging as verdant areas of exploration on the road to discovering cancer9s Achilles heel. One of the distinguishing and near-universal hallmarks of cancer growth is hypoxia. Unregulated cellular proliferation leads to formation of cellular masses ...
Cited By19
Newest
Published on May 1, 2019in Journal of Drug Delivery Science and Technology2.61
Srinivasan Ayyanaar4
Estimated H-index: 4
,
Mookkandi Palsamy Kesavan8
Estimated H-index: 8
+ 3 AuthorsG. Rajagopal10
Estimated H-index: 10
Abstract Non-selectivity of drugs used in the treatment of cancer inevitably leads to destruction of rapidly dividing normal healthy cells. In order to overcome this limitation a wide variety of targeted drug delivery systems have been reported. Among them, microparticulate drug delivery is a one of the most efficient routes and lacks responsiveness to external stimuli. In order to overcome this limitation a new drug delivery system based on reactive oxygen species (ROS) sensitive tween 80 coate...
Published on Feb 18, 2019in International Journal of Molecular Sciences4.18
Xi Li2
Estimated H-index: 2
,
Qiao Zhang4
Estimated H-index: 4
+ 9 AuthorsBowen Zhao
Oxidative stress is the main pathogenesis of diabetic microangiopathy, which can cause microvascular endothelial cell damage and destroy vascular barrier. In this study, it is found that carnosol protects human microvascular endothelial cells (HMVEC) through antioxidative mechanisms. First, we measured the antioxidant activity of carnosol. We showed that carnosol pretreatment suppressed tert-butyl hydroperoxide (t-BHP)-induced cell viability, affected the production of lactate dehydrogenase (LDH...
Published on Sep 1, 2017in Food Chemistry5.40
Wenjie Jian4
Estimated H-index: 4
(SCAU: South China Agricultural University),
Liying Tu1
Estimated H-index: 1
(JMU: Jimei University)
+ 3 AuthorsYuanming Sun20
Estimated H-index: 20
(SCAU: South China Agricultural University)
Abstract Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100 kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100 kGy. We investigated the protective effect of 100 kGy-KGM against H 2 O 2 induced oxidative damage in LO2 cells. Our results demonstrated that...
Published on Apr 10, 2017in Frontiers in Endocrinology3.63
Fatma Amri2
Estimated H-index: 2
(Tunis University),
Ikram Ghouili2
Estimated H-index: 2
(Tunis University)
+ 2 AuthorsOlfa Masmoudi-Kouki10
Estimated H-index: 10
(Tunis University)
Oxidative stress plays a major role in triggering astroglial cell death in diverse neuropathological conditions such as ischemia and neurodegenerative diseases. Numerous studies indicate that hemoglobin (Hb) is expressed in both resting and reactive glia cells, but nothing is known regarding a possible role of hemoglobin on astroglial cells survival. Thus, the purpose of the present study was to investigate the potential glioprotective effect of Hb on hydrogen peroxide (H2O2)-induced oxidative s...
Published on Jan 2, 2017in Journal of Receptors and Signal Transduction1.63
Mahesh Ramalingam6
Estimated H-index: 6
(Kyung Hee University),
Sung-Jin Kim8
Estimated H-index: 8
(Kyung Hee University)
AbstractParkinson’s disease (PD) is the second most common neurodegenerative disorder after Alzheimer’s disease (AD) associated with mitochondrial dysfunction mediated by oxidative stress. Astrocytes regulate neuronal function via the modulation of synaptic transmission and plasticity, secretion of growth factors, uptake of neurotransmitters, and regulation of extracellular ion concentrations and metabolic support of neurons. Therefore, this study was undertaken to investigate the mechanism of a...
Published on Oct 1, 2016in Neuroscience Letters2.17
Mahesh Ramalingam6
Estimated H-index: 6
(Kyung Hee University),
Sung-Jin Kim8
Estimated H-index: 8
(Kyung Hee University)
Abstract Parkinson’s disease (PD) is the second most common neurodegenerative disease in the elderly caused by dopaminergic neuronal cell death. Human neuroblastoma SH-SY5Y cells differentiated by retinoic acid have been used to study the in vitro PD model induced by 1-methyl-4-phenyl pyridinium (MPP + ). In this study, pretreatment of insulin inhibited MPP + -induced cell membrane damages, which also inhibited the Cox-2 and α-synuclein levels. In addition, MPP + and/or insulin enhanced the auto...
Jin Huang1
Estimated H-index: 1
(HUST: Huazhong University of Science and Technology),
Chi Zhou1
Estimated H-index: 1
(HUST: Huazhong University of Science and Technology)
+ 3 AuthorsShenghong Liu3
Estimated H-index: 3
(HUST: Huazhong University of Science and Technology)
Hydrogen peroxide (H2O2) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene (C60) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative (C60-GSH) was successfully synthesized, and its beneficial roles in prot...
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