Immunopurification of a rape (Brassica napus L.) seedling lipase.

Volume: 3, Issue: 11, Pages: 356 - 365
Published: Jan 1, 2009
Abstract
L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain contaminating proteins detectable by SDS-PAGE and HPLC analysis. The specific activity of the purified preparation was increased about 1950 times, with an overall yield of 35%. The rapeseed lipase...
Paper Details
Title
Immunopurification of a rape (Brassica napus L.) seedling lipase.
Published Date
Jan 1, 2009
Volume
3
Issue
11
Pages
356 - 365
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