Original paper
Rehydration Driven RNAi: A Novel Approach for Effectively Delivering dsRNA to Mosquito Larvae
Abstract
The soft bodies and aquatic habitats of mosquito larvae pose a challenge for applying standard RNA-interference techniques to silence expression of target genes. Here we describe a novel technique for delivering double-stranded RNA into mosquito larvae by exploiting the larva's dehydration tolerance. Larvae were dehydrated in a NaCl solution and then rehydrated in water containing double-stranded RNA. Using larvae of Culex pipiens (L.) we...
Paper Details
Title
Rehydration Driven RNAi: A Novel Approach for Effectively Delivering dsRNA to Mosquito Larvae
Published Date
Jan 1, 2012
Volume
49
Issue
1
Pages
215 - 218
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Notes
History