A Targetron System for Gene Targeting in Thermophiles and Its Application in Clostridium thermocellum

Background Targetrons are gene targeting vectors derived from mobile group II introns. They consist of an autocatalytic intron RNA (a “ribozyme”) and an intron-encoded reverse transcriptase, which use their combined activities to achieve highly efficient site-specific DNA integration with readily programmable DNA target specificity. Methodology/Principal Findings Here, we used a mobile group II intron from the thermophilic cyanobacterium...