Studies on Thermolysin

1. Thermolysin was rapidly inactivated by rose bengal-catalyzed photooxidation at pH 8.0 and 37°C. The rate of inactivation was pH-dependent and became slower at lower pH values, suggesting the involvement of some histidine residues in the inactivation. 2. Changes in amino acid composition occurred only with some histidine, trypto-phan and tyrosine residues. The enzyme appeared to be inactivated almost in parallel with the loss of one or two...