Effects of 4 weight-loss diets differing in fat, protein, and carbohydrate on fat mass, lean mass, visceral adipose tissue, and hepatic fat: results from the POUNDS LOST trial

Published on Mar 1, 2012in The American Journal of Clinical Nutrition6.57
· DOI :10.3945/ajcn.111.026328
Russell J. de Souza35
Estimated H-index: 35
George A. Bray114
Estimated H-index: 114
+ 6 AuthorsSteven R. Smith71
Estimated H-index: 71
Background: Weight loss reduces body fat and lean mass, but whether these changes are influenced by macronutrient composition of the diet is unclear. Objective: We determined whether energy-reduced diets that emphasize fat, protein, or carbohydrate differentially reduce total, visceral, or hepatic fat or preserve lean mass. Design: In a subset of participants in a randomized trial of 4 weightloss diets, body fat and lean mass (n = 424; by using dual-energy X-ray absorptiometry) and abdominal and hepatic fat (n = 165; by using computed tomography) were measured after 6 mo and 2 y. Changes from baseline were compared between assigned amounts of protein (25% compared with 15%) and fat (40% compared with 20%) and across 4 carbohydrate amounts (35% through 65%). Results: At 6 mo, participants lost a mean (6SEM) of 4.2 6 0.3 kg (12.4%) fat and 2.1 6 0.3 kg (3.5%) lean mass (both P , 0.0001 compared with baseline values), with no differences between 25% and 15% protein (P 0.10), 40% and 20% fat (P 0.34), or 65% and 35% carbohydrate (P 0.27). Participants lost 2.3 6 0.2 kg (13.8%) abdominal fat: 1.5 6 0.2 kg (13.6%) subcutaneous fat and 0.9 6 0.1 kg (16.1%) visceral fat (all P , 0.0001 compared with baseline values), with no differences between the diets (P 0.29). Women lost more visceral fat than did men relative to total-body fat loss. Participants regained ;40% of these losses by 2 y, with no differences between diets (P 0.23). Weight loss reduced hepatic fat, but there were no differences between groups (P 0.28). Dietary goals were not fully met; self-reported contrasts were closer to 2% protein, 8% fat, and 14% carbohydrate at 6 mo and 1%, 7%, and 10%, respectively, at 2 y. Conclusion: Participants lost more fat than lean mass after consumption of all diets, with no differences in changes in body composition, abdominal fat, or hepatic fat between assigned macronutrient amounts. This trial was registered at as NCT00072995. Am J Clin Nutr 2012;95:614‐25.
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#1Maria Cristina Elias (UNIFESP: Federal University of São Paulo)H-Index: 2
#2Edison Roberto Parise (UNIFESP: Federal University of São Paulo)H-Index: 13
Last.João Prola Netto (UNIFESP: Federal University of São Paulo)H-Index: 1
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#1Donald K. Layman (UIUC: University of Illinois at Urbana–Champaign)H-Index: 33
#2Ellen M. Evans (Urbana University)H-Index: 34
Last.Penny M. Kris-Etherton (PSU: Pennsylvania State University)H-Index: 88
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