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Crystallization of biological macromolecules from flash frozen samples on the Russian Space Station Mir.

Published on Nov 20, 1996in Biotechnology and Bioengineering4.26
· DOI :10.1002/(SICI)1097-0290(19961120)52:4<449::AID-BIT1>3.0.CO;2-P
Stanley Koszelak12
Estimated H-index: 12
(UCR: University of California, Riverside),
Cathy Leja1
Estimated H-index: 1
(UCR: University of California, Riverside),
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Abstract
One hundred eighty-three flash frozen, liquid–liquid diffusion and batch method protein and virus crystallization samples were launched aboard the Space Shuttle Discovery on June 27 (STS-71) and transferred to the Russian Space Station Mir on July 1, 1995. They were returned to earth November 20, 1995 (STS-74). Subsequent examination showed that of the 19 types of proteins and viruses investigated, 17 were crystallized during the period on Mir. The experiment demonstrates the utility of this very simple and inexpensive approach for the crystallization of biological macromolecules in space over extended time periods. The distribution of crystals among the three types of containers used indicated small samples yielded results equal or better than larger samples and that long diffusion path lengths were clearly better. Distribution of crystals within the container tubes showed a striking gradient of quality and size that indicated long, narrow tubes yield superior crystals, as predicted from other work based on crystallization in capillaries. © 1996 John Wiley & Sons, Inc.
  • References (23)
  • Citations (24)
References23
Newest
#1A Malkin (UCR: University of California, Riverside)H-Index: 18
#2Yu. G. Kuznetsov (UCR: University of California, Riverside)H-Index: 19
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 5 authors...
#1Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
#2A Malkin (UCR: University of California, Riverside)H-Index: 18
Last.Yurii G. Kuznetsov (UCR: University of California, Riverside)H-Index: 4
view all 3 authors...
#1Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
#2John Day (UCR: University of California, Riverside)H-Index: 15
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 5 authors...
#1John Day (UCR: University of California, Riverside)H-Index: 15
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
#1Koji Asano (Fujitsu)H-Index: 3
#2Shozo Fujita (Fujitsu)H-Index: 12
Last.Yukio Mitsui (Nagaoka University of Technology)H-Index: 28
view all 4 authors...
#1Robert Dipl Ing Bosch (Dornier Flugzeugwerke)H-Index: 4
#2Peter Lautenschlager (Dornier Flugzeugwerke)H-Index: 5
Last.Juergen Dr Ing Stapelmann (Dornier Flugzeugwerke)H-Index: 3
view all 4 authors...
#1Roland K. Strong (MIT: Massachusetts Institute of Technology)H-Index: 5
#2Barry L. Stoddard (MIT: Massachusetts Institute of Technology)H-Index: 59
Last.Gregory K. Farber (MIT: Massachusetts Institute of Technology)H-Index: 22
view all 4 authors...
Cited By24
Newest
#1V. I. Strelov (RAS: Russian Academy of Sciences)H-Index: 4
#2I. P. Kuranova (RAS: Russian Academy of Sciences)H-Index: 13
Last.A. E. Voloshin (RAS: Russian Academy of Sciences)H-Index: 10
view all 4 authors...
#1Joseph R. Luft (UB: University at Buffalo)H-Index: 28
#2Jennifer R. Wolfley (Hauptman-Woodward Medical Research Institute)H-Index: 9
Last.Michael G. Malkowski (UB: University at Buffalo)H-Index: 23
view all 11 authors...
#1Russell A. Judge (MSFC: Marshall Space Flight Center)H-Index: 13
#2Edward H. Snell (MSFC: Marshall Space Flight Center)H-Index: 23
Last.Mark J. van der Woerd (MSFC: Marshall Space Flight Center)H-Index: 9
view all 3 authors...
#1Rachel R. Potter (MSFC: Marshall Space Flight Center)H-Index: 1
#2Young-Soo Hong (BAE Systems)H-Index: 1
Last.Ewa Ciszak (UAH: University of Alabama in Huntsville)H-Index: 7
view all 3 authors...
View next paperPreliminary investigations of protein crystal growth using the Space Shuttle