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Crystallization of biological macromolecules from flash frozen samples on the Russian Space Station Mir.

Published on Nov 20, 1996in Biotechnology and Bioengineering4.26
· DOI :10.1002/(SICI)1097-0290(19961120)52:4<449::AID-BIT1>3.0.CO;2-P
S. Koszelak12
Estimated H-index: 12
(UCR: University of California, Riverside),
Cathy Leja1
Estimated H-index: 1
(UCR: University of California, Riverside),
Alexander McPherson51
Estimated H-index: 51
(UCR: University of California, Riverside)
Abstract
One hundred eighty-three flash frozen, liquid–liquid diffusion and batch method protein and virus crystallization samples were launched aboard the Space Shuttle Discovery on June 27 (STS-71) and transferred to the Russian Space Station Mir on July 1, 1995. They were returned to earth November 20, 1995 (STS-74). Subsequent examination showed that of the 19 types of proteins and viruses investigated, 17 were crystallized during the period on Mir. The experiment demonstrates the utility of this very simple and inexpensive approach for the crystallization of biological macromolecules in space over extended time periods. The distribution of crystals among the three types of containers used indicated small samples yielded results equal or better than larger samples and that long diffusion path lengths were clearly better. Distribution of crystals within the container tubes showed a striking gradient of quality and size that indicated long, narrow tubes yield superior crystals, as predicted from other work based on crystallization in capillaries. © 1996 John Wiley & Sons, Inc.
  • References (23)
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References23
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#1Arnaud DucruixH-Index: 9
#2R. GiegH-Index: 1
Giege and Ducruix: An introduction to the crystallogenesis of biological macromolecules Lorber and Giege: Biochemical aspects and handling of macromolecular solutions and crystals Berne, Doublie and Carter, Jr: Molecular biology for structural biology Carter, Jr: Experimental design, quantitive analysis, and the cartography of crystal growth Ducruix and Giege: Methods of crystallization Robert et al: Crystallization in gels and related methods Stura: Seeding techniques Dock-Bregeon, Moras and Gi...
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#2Yu. G. Kuznetsov (UCR: University of California, Riverside)H-Index: 19
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The growth of six protein and virus crystals was investigated in situ using atomic force microscopy. Most of the crystals grew principally on steps generated by two-dimensional nucleation on surfaces though some grew by development of spiral dislocations. Apoferritin grew by a rarely encountered mechanism, normal growth, usually associated only with melt or vapour phase crystallization. Cubic crystals of satellite tobacco mosaic virus (STMV) grew, at moderate to high levels of supersaturation, b...
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Thanks to Bob Cudney, Hampton Research, Laguna Hills, CA, USA for kindly providing Figure 11Figure 11. The research described here was supported by grants and contracts from the National Aeronautics and Space Administration.
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