Anomalous signal of solvent bromides used for phasing of lysozyme a aEdited by I. A. Wilson

The anomalous signal of bromide ions, present in the crystal structure of tetragonal hen egg-white lysozyme through the substitution of NaCl by NaBr in the crystallization medium, was used for phasing of X-ray data collected to 1.7 Å resolution with a wavelength near the absorption edge of bromine. Phasing of a single wavelength data set, based purely on anomalous δf″″ contribution, led to easily interpretable electron density, equivalent to the...