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Evidence for a satellite RNA associated naturally with the U5 strain and experimentally with the U1 strain of tobacco mosaic virus

Published on Sep 1, 1986in Journal of General Virology2.81
· DOI :10.1099/0022-1317-67-9-1875
Rodrigo A. Valverde20
Estimated H-index: 20
(UCR: University of California, Riverside),
J.A. Dodds5
Estimated H-index: 5
(UCR: University of California, Riverside)
Abstract
Summary Isolates of tobacco mosaic virus strain U5 (TMV-U5) from native Nicotiana glauca plants induced the accumulation of a dsRNA (mol. wt. 0.6 × 106) in infected plants that was more abundant than the replicative form (RF) dsRNA of TMV (mol. wt. 4.3 × 106). Some but not all subcultures of such a field isolate obtained from single local lesions on N. tabacum cv. Xanthi-nc, had lost the ability to induce the 0.6 × 106 mol. wt. dsRNA. Co-inoculation experiments in N. silvestris established that the dsRNA could accumulate in plants infected with TMV-U1, but to a lesser extent than when associated with TMV-U5. A ssRNA (mol. wt. 0.3 × 106) was isolated from plants containing the dsRNA. This was not infectious by itself but became so when associated with TMV-U5 or TMV-U1, and then induced the accumulation of the 0.6 × 106 dsRNA. Plants infected with TMV-U5 isolates which did or did not induce the 0.6 × 106 dsRNA had identical symptoms. The host range of the 0.3 × 106 ssRNA was the same as that of the TMV strain with which it was associated in each of the 20 experimental hosts tested. Complementary DNA transcribed from purified 0.6 × 106 dsRNA did not hybridize with RF and other dsRNAs of TMV-U5, TMV-U1, tobacco necrosis virus, potato virus X, citrus tristeza virus, and cucumber mosaic virus + CARNA 5, but the cDNA did hybridize with the 0.6 × 106 mol. wt. dsRNA and the 0.3 × 106 mol. wt. ssRNA found only in plants containing this dsRNA. The results indicate that the 0.6 × 106 dsRNA is the RF of a satellite RNA of TMV. Purified nucleoprotein from plants infected with TMV-U5 and the satellite RNA were infectious for the satellite RNA, but the nature of encapsidation of the satellite RNA has yet to be determined.
  • References (28)
  • Citations (37)
References28
Newest
#1J. A. Dodds (UCR: University of California, Riverside)H-Index: 6
#2S.Q. Lee (UCR: University of California, Riverside)H-Index: 1
Last.M. Tiffany (UCR: University of California, Riverside)H-Index: 1
view all 3 authors...
#1G.D. Gustafson (Purdue University)H-Index: 4
#2Joel J. Milner (Purdue University)H-Index: 4
Last.A.O. Jackson (Purdue University)H-Index: 9
view all 6 authors...
Cited By37
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#1Aurora Fraile (UPM: Technical University of Madrid)H-Index: 30
#2Fernando García-Arenal (UPM: Technical University of Madrid)H-Index: 39
#1S. Khankhum (Louisiana State University Agricultural Center)H-Index: 1
#2Cesar Escalante (Louisiana State University Agricultural Center)H-Index: 1
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
view all 4 authors...
#1Venkatesh Sivanandam (UCR: University of California, Riverside)H-Index: 2
#2D. M. Mathews (UCR: University of California, Riverside)H-Index: 8
Last.A.L.N. Rao (UCR: University of California, Riverside)H-Index: 25
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#2John Day (UCI: University of California, Irvine)H-Index: 10
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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#1R. F.C. Hull (JIC: John Innes Centre)H-Index: 45
#1Christelle Desnues (CNRS: Centre national de la recherche scientifique)H-Index: 24
#2Mickaël Boyer (CNRS: Centre national de la recherche scientifique)H-Index: 11
Last.Didier Raoult (CNRS: Centre national de la recherche scientifique)H-Index: 138
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#1Yuri G. Kuznetsov (UAH: University of Alabama in Huntsville)H-Index: 1
#2Jeffrey J. Dowell (UAH: University of Alabama in Huntsville)H-Index: 1
Last.Alexander McPherson (UAH: University of Alabama in Huntsville)H-Index: 2
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View next paperInteractions between satellite tobacco mosaic virus, helper tobamoviruses, and their hosts