‘De novo’ sequencing of Atlantic cod vitellogenin tryptic peptides by matrix‐assisted laser desorption/ionization quadrupole time‐of‐flight tandem mass spectrometry: similarities with haddock vitellogenin

Vitellogenin is a protein produced by the liver of oviparous animals in response to circulating estrogens. The amino acid sequence of vitellogenin from Atlantic cod ( Gadus morhua ) has not yet been determined. In this study Atlantic cod vitellogenin was characterized using a ‘bottom‐up’ mass spectrometric approach. Vitellogenin synthesis was induced ‘ in vivo ’ with β ‐estradiol, and subjected to trypsin digestion for characterization by...