Scanning inverse fluorescence correlation spectroscopy
Abstract
Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross- and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm...
Paper Details
Title
Scanning inverse fluorescence correlation spectroscopy
Published Date
May 22, 2014
Journal
Volume
22
Issue
11
Pages
13073 - 13073
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