Dietary lysine requirement of large yellow croaker (Pseudosciaena crocea, Richardson 1846) larvae

A 30-day feeding experiment was conducted to estimate the lysine requirement of large yellow croaker larvae (2.75 ± 0.11 mg). Six isonitrogenous (509.5–519.7 g kg−1 crude protein) and isoenergetic (22.3–22.5 kJ g−1 energy) microdiets containing graded levels of lysine·HCl ranging from 24.8 to 41.0 g kg−1 diet in placement of glycine and glutamic acid were formulated. Mixture of crystalline amino acids (MAA) was supplemented to simulate the amino...