Activation function 1 domain plays a negative role in dimerization of estrogen receptor beta

Transcriptional potential of estrogen receptor beta (ERbeta) depends on the ligand binding and subsequent dimerization of the receptor protein. In order to examine the role of N-terminally located activation function 1 (AF-1) protein domain in the dimerization process of ERbeta, we used yeast SOS-Recruitment System (SRS). Two variants of ERbeta protein were expressed in the yeast cells: full length receptor and a truncated form, lacking AF-1. We...