Singlet oxygen imaging inArabidopsis thalianaleaves under photoinhibition by excess photosynthetically active radiation

Arabidopsis thaliana leaves were infiltrated with DanePy (3‐( N ‐diethylaminoethyl)‐ N ‐dansyl)aminomethyl‐2,5‐dihydro‐2,2,5,5‐tetramethyl‐1 H ‐pyrrole), a double, fluorescent and spin sensor of singlet oxygen. DanePy fluorescence was imaged by laser scanning microscopy. We found that DanePy penetrated into chloroplasts but did not alter the functioning of the photosynthetic electron transport as assessed by chlorophyll fluorescence induction....