High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system
Abstract
Cell-free protein synthesis systems can synthesize proteins with high speed and accuracy, but produce only a low yield because of their instability over time. Here we review our recent advances in a cell-free protein synthesis system prepared from wheat embryos. We first addressed and resolved the source of the instability of existing systems in light of endogenous ribosome-inactivating proteins. We found that conventional wheat germ extracts...
Paper Details
Title
High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system
Published Date
Nov 1, 2003
Journal
Volume
21
Issue
8
Pages
695 - 713
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