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Bioinformatics Analysis of Zinc-Alpha2-Glycoprotein,a Potential Serological Marker for Hepatocellualr Carcinoma

Published on Sep 1, 2011in Advanced Materials Research
· DOI :10.4028/WWW.SCIENTIFIC.NET/AMR.340.390
Fei Wang3
Estimated H-index: 3
(Tianjin Medical University),
Yu Geng1
Estimated H-index: 1
+ 1 AuthorsXinGeng (Tianjin Medical University)
Abstract
To Analyze the structure and function of Zinc-alpha-2-glycoprotein (ZAG), a highly expressed serum protein in the patients with hepatocellular carcinoma (HCC). The serum proteome of the patients from HCC, liver cirrhosis and healthy donors were separated and identified by two-dimensional electrophoresis and MALDI-TOF-MS. RT-PCR and Western blotting analysis were used to confirm ZAG highly expressed in HCC. Bioinformatics technologies were used to analyze the structure character of ZAG protein. Five differentially expressed proteins were identified. Albumin, Serotransferrin,CD5 antigen-like precursor ( IgM - associated peptide) were down-regulated in HCC, ZAG and Ig gamma-1 chain C region were up-regulated in HCC. Zinc-alpha-2-glycoprotein (ZAG), a lipid mobilizing factor, is a member of the major histocompatibility complex (MHC) class I family of protein. ZAG protein might be a potential serological biomarker for HCC early diagnosis.
  • References (11)
  • Citations (1)
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References11
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#1Tomas Mracek (University of Liverpool)H-Index: 7
#2Q. Ding (University of Liverpool)H-Index: 4
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Introduction Zinc-a2-glycoprotein (ZAG) is a novel adipokine, which may act locally to influence adipocyte metabolism. This study assessed the effect of increased adiposity on ZAG expression in adipose tissue in human subjects. The study also examined the association between ZAG and adiponectin expression in human adipose tissue, and whether ZAG modulates adiponectin secretion by human adipocytes. Methods Adipose tissue (visceral and subcutaneous) was collected from human subjects with a wide ra...
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#1Xin-Qing PengH-Index: 1
#2Fei Wang (Tianjin Medical University)H-Index: 3
Last. Wei-Ming Zhang (Tianjin Medical University)H-Index: 1
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Tumor proteomics apply proteomics techniques to tumor biological research, mainly by screening candidate biomarkers for early tumor diagnosis, prognosis and treatment. Hepatocellular carcinoma (HCC) is a type of malignant tumor with one of the highest death rates in the world. With the advent of the post-genomic age, tumor biological research developing the technology of proteomics has become a major focus of researchers. The discovery of novel candidate biomarkers is one of crucial problems for...
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#1Feng Ying Gong (Peking Union Medical College Hospital)H-Index: 4
#2S. J. ZhangH-Index: 1
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Zinc-α2-glycoprotein (ZAG) was found to influence lipolysis in adipose tissue and has recently been proposed as a candidate factor in the regulation of body weight. To elucidate the association of serum ZAG level with body weight and percentage of body fat in normal, obese subjects and high-fat diet (HFD)-induced obese mice. The relationship between serum ZAG and obesity-related parameters was studied in 44 human subjects and 36 mice fed standard food and HFD. Furthermore, the effects of ZAG ove...
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Context: Zinc-α2-glycoprotein (ZAG) is a 40-kDa circulating glycoprotein secreted from the liver and adipose tissues. Animal studies have demonstrated the role of ZAG as a lipid-mobilizing factor involved in regulating lipid metabolism and adiposity. However, the clinical relevance of these findings remains to be established. Objective: This study aimed to address the relationship of serum ZAG levels with adiposity and cardiometabolic risk factors in humans. Design and Setting: A total of 258 Ch...
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Hepatocellular carcinoma is a malignant tumor causing one of the highest death rates in the world. Viral hepatitis, hepatic fibrosis and hepatocirrhosis etc. are some of the most important causes of hepatocellular carcinoma. With the advent of the post-genomic age, studying carcinoma and some related diseases using the developing technology of proteomics has become a major focus of researchers. This article is a review of the application of proteomics to study hepatocellular carcinoma and some r...
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