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Reinduction of T-Type Calcium Channels by Endothelin-1 in Failing Hearts In Vivo and in Adult Rat Ventricular Myocytes In Vitro

Published on Nov 18, 2003in Circulation23.05
· DOI :10.1161/01.CIR.0000096484.03318.AB
Toshiaki Izumi9
Estimated H-index: 9
(Kyoto University),
Yasuki Kihara40
Estimated H-index: 40
(Kyoto University)
+ 7 AuthorsAkinori Noma37
Estimated H-index: 37
(Kyoto University)
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Abstract
Background— In ventricular myocardium, the T-type Ca2+ current (ICa,T), which is temporarily observed during fetal and neonatal periods, has been shown to reappear in failing/remodeling hearts. However, its pathophysiological regulation has not been elucidated. Methods and Results— We utilized Dahl salt-sensitive (DS) rats with hypertension at the stage of concentric left ventricular (LV) hypertrophy (11 weeks old, LVH) and at the heart failure stage (16 to 18 weeks old, CHF). Some were treated with bosentan (100 mg/kg per day) during the period from LVH to CHF. In LVH, neither the presence of ICa,T (measured in the freshly isolated LV myocytes) nor an increase in α-1G mRNA expression were detected. This condition was associated with increases in tissue angiotensin II (AII) but not with endothelin (ET)-1 peptides. In contrast, in CHF, when the tissue AII remained elevated and ET-1 de novo increased, ICa,T was recorded in most of the cells (−0.87±0.18 pA/pF at −30 mV, P<0.01 versus LVH). This was associate...
  • References (29)
  • Citations (59)
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References29
Newest
Published on Jul 31, 2001in Circulation23.05
Yoshitaka Iwanaga30
Estimated H-index: 30
,
Yasuki Kihara40
Estimated H-index: 40
+ 4 AuthorsSasayama S67
Estimated H-index: 67
Background In view of their mutual crosstalk, the roles of angiotensin II (Ang II) and endothelin-1 (ET-1) in the myocardium are assumed to be synergistic and supplemental. Methods and Results In the phase of compensated left ventricular (LV) hypertrophy of Dahl salt-sensitive rats, Ang II peptide and the ACE mRNA in the LV were increased by 1.6- and 3.8-fold, respectively. In contrast, ET-1 peptide and the preproET-1 mRNA remained unchanged. In subsequent congestive heart failure (CHF), Ang II ...
Published on Apr 1, 2001in Journal of Molecular and Cellular Cardiology5.05
Wataru Hayashida11
Estimated H-index: 11
(Kyoto University),
Yasuki Kihara40
Estimated H-index: 40
(Kyoto University)
+ 3 AuthorsSasayama S67
Estimated H-index: 67
(Kyoto University)
Abstract Mitogen-activated protein kinases (MAPKs) are involved in the early development of cardiac hypertrophy, but their roles in chronic left ventricular hypertrophy (LVH) are unclear. We studied the angiotensin (Ang) II-induced cardiac MAPK activation of the hypertensive Dahl salt-sensitive (DS) rats in the subacute developing LVH stage, the chronic compensated LVH stage, and the congestive heart failure (CHF) stage. In the isolated, coronary-perfused heart preparation, Ang II infusion (1×10...
Published on Jun 1, 2000in Cardiovascular Research7.01
Boyu Huang10
Estimated H-index: 10
,
Dayi Qin7
Estimated H-index: 7
+ 2 AuthorsNabil El-Sherif63
Estimated H-index: 63
Objective: T-type Ca2+ currents ( I Ca-T) are present in neonatal rat myocytes but is not detected in adult ventricular myocytes. The present study was designed to investigate the expression of the T-type Ca2+ channel gene and current in post-infarction remodeled hypertrophied rat left ventricle (LV). Methods: We compared the expression of T-type Ca2+ channel gene α-1G in neonatal rat LV, in adult sham-operated LV and remodeled hypertrophied LV 3 to 4 weeks post-myocardial infarction (MI) using ...
Published on May 5, 2000in Journal of Biological Chemistry
Tatsuya Morimoto28
Estimated H-index: 28
(Kyoto University),
Koji Hasegawa45
Estimated H-index: 45
(Kyoto University)
+ 4 AuthorsShigetake Sasayama6
Estimated H-index: 6
(Kyoto University)
Abstract The expression of endothelin-1 (ET-1) in cardiac myocytes is markedly induced during the development of heart failurein vivo and by stimulation with the α1-adrenergic agonist phenylephrine in culture. Although recent studies have suggested a role for cardiac-specific zinc finger GATA factors in the transcriptional pathways that modulate cardiac hypertrophy, it is unknown whether these factors are also involved in cardiac ET-1 transcription and if so, how these factors are modulated duri...
Published on Feb 22, 2000in Circulation23.05
T. Barry Levine1
Estimated H-index: 1
,
Peter J. L. M. Bernink6
Estimated H-index: 6
+ 10 AuthorsAlon Marmor14
Estimated H-index: 14
Background—Calcium antagonists have proved disappointing in long-term congestive heart failure (CHF) studies. Mibefradil, a new calcium antagonist that selectively blocks T-type calcium channels, has been shown to be an effective antihypertensive, antianginal, and anti-ischemic agent, and because of its different mechanism of action, it may be beneficial as adjunct therapy in CHF patients. Methods and Results—This multicenter, randomized, double-blind study compared mibefradil with placebo as ad...
Published on Sep 1, 1999in Journal of Molecular and Cellular Cardiology5.05
Marı´a Luisa Martı´nez1
Estimated H-index: 1
(Complutense University of Madrid),
Marı´a Puy Heredia1
Estimated H-index: 1
(Complutense University of Madrid),
Carmen Delgado19
Estimated H-index: 19
(Complutense University of Madrid)
Abstract In this study we examined the existence of T-type Ca 2+ current in ventricular myocytes isolated from rats with pressure-overload hypertrophy. The whole-cell clamp technique was used to record Ca 2+ currents in enzymatically dissociated ventricular cells. T- and L-type Ca 2+ currents were separated by applying voltage steps to different test potentials from a holding potential of −80 mV and −50 mV. T-type Ca 2+ current was defined as the difference between the currents from the two hold...
Published on Nov 10, 1998in Circulation23.05
Yoshitaka Iwanaga30
Estimated H-index: 30
(Kyoto University),
Yasuki Kihara40
Estimated H-index: 40
(Kyoto University)
+ 5 AuthorsShigetake Sasayama9
Estimated H-index: 9
(Kyoto University)
Background —To investigate whether endogenous ET-1 participates in an adaptive process of left ventricular hypertrophy (LVH) or a maladaptive process from LVH to congestive heart failure (CHF), we used a Dahl salt-sensitive (DS) rat model, in which systemic hypertension caused compensated concentric LVH at the age of 11 weeks followed by marked LV dilatation and global hypokinesis at the age of 17 weeks. Methods and Results —By specific sandwich enzyme immunoassay, serum and myocardial ET-1 leve...
Published on Jun 1, 1998in Journal of Molecular and Cellular Cardiology5.05
Weinong Guo12
Estimated H-index: 12
(Nagoya University),
Kaichiro Kamiya33
Estimated H-index: 33
(Nagoya University)
+ 1 AuthorsJunji Toyama37
Estimated H-index: 37
(Nagoya University)
Abstract The expression of T-type Ca 2+ current (I Ca,T ) has been reported to change during postnatal heart development and myocardial hypertrophy, which are characterized respectively by the arrest of the cell cycle soon after birth and a switching on of DNA synthesis in the terminally differentiated cardiac myocytes. The hypothesis that there are cell cycle-related changes in cardiac Ca 2+ channel expression was tested by performing whole-cell voltage-clamp recording and BromodeoxyUridine (Br...
Published on Apr 1, 1998in The Journal of Physiology
Karin R. Sipido47
Estimated H-index: 47
(Katholieke Universiteit Leuven),
Edward Carmeliet41
Estimated H-index: 41
(Katholieke Universiteit Leuven),
F. Van de Werf112
Estimated H-index: 112
(Katholieke Universiteit Leuven)
1We have investigated whether Ca2+ entry through T-type Ca2+ channels participates in triggering Ca2+ release from the sarcoplasmic reticulum (SR) in single guinea-pig ventricular myocytes (whole-cell voltage clamp, K5fura-2 as [Ca2+]i indicator; all monovalent cations replaced by impermeant ions to record uncontaminated Ca2+ currents; T= 23 or 36 °C). 2T-type Ca2+ currents were elicited from a holding potential of -90 mV during steps to -50 to -20 mV. For steps to -50 mV, very small [Ca2+]i tra...
Published on Feb 1, 1998in Nature43.07
Edward Perez-Reyes57
Estimated H-index: 57
(Loyola University Medical Center),
Leanne L. Cribbs33
Estimated H-index: 33
(Loyola University Medical Center)
+ 6 AuthorsJung-Ha Lee9
Estimated H-index: 9
(Loyola University Medical Center)
The molecular diversity of voltage-activated calcium channels was established by studies showing that channels could be distinguished by their voltage-dependence, deactivation and single-channel conductance1,2,3. Low-voltage-activated channels are called ‘T’ type because their currents are both transient (owing to fast inactivation) and tiny (owing to small conductance)2. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and res...
Cited By59
Newest
Published on Mar 21, 2019in PLOS ONE2.78
Uppala Radhakrishna3
Estimated H-index: 3
(Oakland University),
Samet Albayrak3
Estimated H-index: 3
(WSU: Wayne State University)
+ 9 AuthorsChittibabu Guda21
Estimated H-index: 21
Published on Mar 21, 2019in bioRxiv
Uppala Radhakrishna3
Estimated H-index: 3
(Oakland University),
Samet Albayrak3
Estimated H-index: 3
(WSU: Wayne State University)
+ 9 AuthorsNitish K. Mishra10
Estimated H-index: 10
(UNMC: University of Nebraska Medical Center)
Ventricular Septal Defect (VSD), the most common congenital heart defect, is characterized by a hole in the septum between the right and left ventricles. The pathogenesis of VSD is unknown in most clinical cases. There is a paucity of data relevant to epigenetic changes in VSD. The placenta is a fetal tissue and is a potentially useful surrogate for the evaluation of fetal organ development. To understand epigenetic mechanisms that may play a role in the development of VSD, a genome-wide DNA met...
Published on Jan 1, 2018
Alexander Kushnir6
Estimated H-index: 6
,
Steven O. Marx37
Estimated H-index: 37
Published on Jan 1, 2018
Alexander Kushnir6
Estimated H-index: 6
,
Steven O. Marx37
Estimated H-index: 37
D. Falcón1
Estimated H-index: 1
(University of Seville),
R. González-Montelongo (University of Seville)+ 3 AuthorsAntonio Castellano19
Estimated H-index: 19
(University of Seville)
Abstract Glucocorticoids are widely used to treat acute and chronic diseases. Unfortunately, their therapeutic use is associated with severe side effects. Glucocorticoids are known to regulate several ion channels in cardiac myocytes, including voltage-dependent Ca 2+ channels. Low-voltage-activated T-type Ca 2+ channels are expressed in ventricular myocytes during the fetal and perinatal period, but are practically absent in the adult. However, these channels can be re-expressed in adult cardio...
Published on Dec 1, 2017in Journal of Cardiovascular Medicine1.43
Matteo Cameli26
Estimated H-index: 26
(UNISI: University of Siena),
Giulia Elena Mandoli4
Estimated H-index: 4
(UNISI: University of Siena)
+ 10 AuthorsKetty Savino5
Estimated H-index: 5
(University of Perugia)
Fang Rao5
Estimated H-index: 5
(Academy of Medical Sciences, United Kingdom),
Yumei Xue5
Estimated H-index: 5
(Academy of Medical Sciences, United Kingdom)
+ 7 AuthorsChun-Yu Deng10
Estimated H-index: 10
(Academy of Medical Sciences, United Kingdom)
Summary Increasing evidence indicates that inflammation contributes to the initiation and perpetuation of atrial fibrillation (AF). Although tumour necrosis factor (TNF)-α levels are increased in patients with AF, the role of TNF-α in the pathogenesis of AF remains unclear. Besides L-type Ca2+ currents (ICa,L), T-type Ca2+ currents (ICa,T) also plays an important role in the pathogenesis of AF. This study was designed to use the whole-cell voltage-clamp technique and biochemical assays to explor...
Published on Jan 1, 2016
Jessica M. Berthiaume9
Estimated H-index: 9
(Case Western Reserve University),
Jonathan A. Kirk15
Estimated H-index: 15
(Johns Hopkins University)
+ 8 AuthorsVivek Rao81
Estimated H-index: 81
(U of T: University of Toronto)
Heart failure (HF) is a highly complex clinical syndrome, culminating from a diversity of environmental and genetic etiologies that compromise pump function, ultimately resulting in insufficient blood flow to the body. Despite the initial disease in the heart, the pathophysiology of HF evolves to affect multiple organ systems throughout the body, primarily the renal, autonomic, vascular, and cardiac system. The structural and functional underpinnings of HF are diverse and accompanied by various ...
View next paperReexpression of T-type Ca2+ channel gene and current in post-infarction remodeled rat left ventricle