Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes
Abstract
Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr locus by zygote injection of two single-guide and Cas9 RNAs. Injection into C57BL/6N wild-type embryos resulted in one completely...
Paper Details
Title
Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes
Published Date
Sep 1, 2014
Journal
Volume
393
Issue
1
Pages
3 - 9
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