Observation of the early stage of insulin crystallization by confocal laser scanning microscopy
Abstract
It is demonstrated that high resolution confocal laser scanning microscopy (CLSM) is a powerful tool for in situ observation and analysis of protein crystal growth. CLSM is used to study the early crystallization stage of Des-ThrB30 human insulin in aqueous solution, under conditions known to lead to monoclinic crystals. A modified batch crystallization method for CLSM purposes is applied which allows the growth behavior of crystallites to be...
Paper Details
Title
Observation of the early stage of insulin crystallization by confocal laser scanning microscopy
Published Date
Nov 1, 2001
Journal
Volume
232
Issue
1-4
Pages
93 - 101
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