Preparation of the immobilized metal affinity membrane with high amount of metal ions and protein adsorption efficiencies

In this study, an approach to prepare immobilized metal affinity membrane (IMAM) with high metal ions and protein adsorption capacities was developed. In the process of coupling epichlorohydrin (EPI) to the regenerated cellulose membrane (RC membrane), NaOH concentration is found to be the most critical. With a lower NaOH concentration, only a minimal amount of EPI reacted to the RC membrane. When NaOH concentration was higher, the membrane was...