Seeking carotenoid pigments in amber-preserved fossil feathers

Published on May 1, 2015in Scientific Reports4.01
· DOI :10.1038/srep05226
Daniel B. Thomas12
Estimated H-index: 12
Paul C. Nascimbene15
Estimated H-index: 15
+ 2 AuthorsHelen F. James28
Estimated H-index: 28
View in Source
Animal colours can be richly informative about aspects of behaviour such as foraging ecology and mate preference. Birds in particular display many striking hues and complex patterns of pigmentation. Examples of plumage adaptations include brightly coloured feathers for enticing potential mates, as well as cryptic patterns that allow a bird to hide in plain sight1,2,3. By analogy with modern birds, the behaviours and habitats of ancient birds and other feathered dinosaurs may be inferred from pigments in fossil plumage. Methods for describing one class of plumage pigment (melanins) have recently been developed for ancient feathers4,5. Modern birds display six chemically-distinct classes of feather pigment, of which carotenoids are the most common after melanins; carotenoids mostly confer yellow, orange and red colours to feathers6,7. The two key challenges for describing carotenoids in ancient plumage are 1) to find an environment that preserves molecular evidence for carotenoids, and 2) to ascertain a technique that provides unequivocal evidence for carotenoids. We focused on feathers in amber because encapsulation within amber may insulate carotenoid molecules against diagenetic alteration, and such preserved pigments could be detected with Raman spectroscopy. Carotenoids have an unequivocal Raman spectral signal that is greatly enhanced by a resonance effect, allowing trace amounts to be detected8. Furthermore, Raman spectroscopy can be performed without sample preparation or destruction. Edwards and colleagues9 showed that inclusions in amber can be studied with minimal or no interference from the amber matrix when Raman spectra are collected with confocal optics and 1064 nm excitation. With conventional Raman microscopy, laser light is channeled through a microscope objective towards a sample, and then scattered light is channeled back through the same objective towards a detector. Light scatters from the entire sample volume that is penetrated by incident light, and the greatest concentration of scattered photons are typically returned from the focal plane of the incident light. Accordingly, light is scattered from both the surface and internal volume of a translucent sample. Confocal Raman microscopy differs from conventional Raman microscopy by the inclusion of a confocal pinhole above the microscope objective. The pinhole allows only the photons scattered from the focal plane to reach the detector. For a translucent sample with an inclusion (e.g. amber containing a fossil feather), the inclusion can be positioned in the focal plane of the incident light and only the light scattered from the inclusion will reach the detector. Hence, confocal Raman microscopy usefully provides chemical information about an inclusion with minimal or no interference from the surface or surrounding matrix. Near infrared excitation wavelengths are typically less sensitive than visible wavelengths when studying pigments with Raman spectroscopy. However, visible excitation wavelengths tend to induce fluorescence when interacting with amber9. Thus, from a signal vs. noise perspective, a Raman spectrum of an inclusion in amber collected with a near infrared wavelength can be substantially more informative than a Raman spectrum of the same inclusion collected with a visible wavelength. A confocal Raman microscope with a near infrared (NIR) laser is thus an ideal tool for seeking carotenoids preserved in amber. We first conducted a pilot study to determine if the carotenoid pigments of a modern feather could be detected through an amber matrix. A yellow and black wing feather from a greenfinch (Carduelis chloris) was placed underneath a polished piece of amber and analysed with Raman spectroscopy. In a second series of experiments, we analysed six fossil feathers preserved in amber using Raman spectroscopy and light microscopy. Evidence for pigmentation in the fossil feathers was sought with Raman spectroscopy, and the preservation of fine-scale morphology was studied with light microscopy. Finally, we collected Raman spectra and scanning electron microscopy images from an ancient feather preserved as a carbonised compression fossil. Compression fossils have previously provided evidence for melanin pigmentation4,5, and here we compared Raman spectral evidence from two types of fossil feather (1: preserved in amber; 2: preserved in lake sediment) with spectra from a modern feather.
Figures & Tables
  • References (18)
  • Citations (17)
#1Daniel B. Thomas (National Museum of Natural History)H-Index: 12
#2Kevin J. McGraw (ASU: Arizona State University)H-Index: 52
Last.Odile Madden (Museum Conservation Institute)H-Index: 5
view all 4 authors...
14 CitationsSource
#1Maria E. McNamara (Yale University)H-Index: 14
#2Derek E. G. Briggs (Yale University)H-Index: 65
Last.Zhengrong Wang (Yale University)H-Index: 21
view all 5 authors...
42 CitationsSource
#1Daniel B. Thomas (Smithsonian Institution)H-Index: 12
#2Cushla M. McGoverin (TU: Temple University)H-Index: 18
Last.Odile Madden (Smithsonian Institution)H-Index: 5
view all 5 authors...
25 CitationsSource
#1Roy A. Wogelius (University of Manchester)H-Index: 23
#2P. M. Manning (University of Manchester)H-Index: 68
Last.Uwe Bergmann (SLAC National Accelerator Laboratory)H-Index: 54
view all 12 authors...
101 CitationsSource
#1Thanh-Lan Gluckman (University of Melbourne)H-Index: 5
#2Gonçalo C. Cardoso (University of Melbourne)H-Index: 20
25 CitationsSource
#1Quanguo Li (AMNH: American Museum of Natural History)H-Index: 7
#2Ke-Qin Gao (PKU: Peking University)H-Index: 21
Last.Richard O. Prum (Yale University)H-Index: 47
view all 9 authors...
156 CitationsSource
#1Howell G. M. Edwards (University of Bradford)H-Index: 45
#2Dennis W. Farwell (University of Bradford)H-Index: 28
Last.Susana E. Jorge Villar (UBU: University of Burgos)H-Index: 20
view all 3 authors...
21 CitationsSource
#1Hartwig SchulzH-Index: 30
#2Malgorzata Baranska (Jagiellonian University)H-Index: 32
Last.Rafal BaranskiH-Index: 18
view all 3 authors...
175 CitationsSource
25 CitationsSource
#1David A. GrimaldiH-Index: 41
#2Michael S. EngelH-Index: 37
Last.Paul C. NascimbeneH-Index: 15
view all 3 authors...
485 CitationsSource
Cited By17
#1Lida Xing (China University of Geosciences)H-Index: 19
#2Ryan C. McKellar (KU: University of Kansas)H-Index: 13
Last.Huijuan Mai (Yunnan University)
view all 5 authors...
#1Arindam Roy (HKU: University of Hong Kong)
#2Michael Pittman (HKU: University of Hong Kong)H-Index: 11
Last.Xing Xu (CAS: Chinese Academy of Sciences)H-Index: 44
view all 5 authors...
#1Michael J. Benton (UoB: University of Bristol)H-Index: 68
#2Danielle DhouaillyH-Index: 25
Last.Maria E. McNamara (UCC: University College Cork)H-Index: 14
view all 4 authors...
2 CitationsSource
#1Ekaterina A. Sidorchuk (RAS: Russian Academy of Sciences)H-Index: 10
#2Andre V. BochkovH-Index: 14
Last.O. F. Chernova (RAS: Russian Academy of Sciences)H-Index: 6
view all 4 authors...
2 CitationsSource
#1Lida Xing (China University of Geosciences)H-Index: 19
#2Pierre F.D. Cockx (University of Regina)H-Index: 3
Last.Jingmai K. O’Connor (CAS: Chinese Academy of Sciences)H-Index: 26
view all 4 authors...
3 CitationsSource
#1Lida Xing (China University of Geosciences)H-Index: 19
#2Michael W. Caldwell (U of A: University of Alberta)H-Index: 32
Last.Ming Bai (CAS: Chinese Academy of Sciences)H-Index: 16
view all 12 authors...
9 CitationsSource
#1Paul A. Selden (KU: University of Kansas)H-Index: 29
#2David Penney (University of Manchester)H-Index: 18
3 CitationsSource
#1Lida Xing (China University of Geosciences)H-Index: 19
#2Ryan C. McKellar (University of Regina)H-Index: 13
Last.Philip J. Currie (U of A: University of Alberta)H-Index: 52
view all 14 authors...
41 CitationsSource
#1Lida Xing (China University of Geosciences)H-Index: 19
#2Ryan C. McKellar (University of Regina)H-Index: 13
Last.Xing Xu (CAS: Chinese Academy of Sciences)H-Index: 44
view all 13 authors...
33 CitationsSource
#1Gustavo M. E. M. Prado (USP: University of São Paulo)H-Index: 2
#2Luiz Eduardo Anelli (USP: University of São Paulo)H-Index: 9
Last.Guilherme Raffaeli Romero (USP: University of São Paulo)H-Index: 3
view all 4 authors...
4 CitationsSource
View next paperTrace Metals as Biomarkers for Eumelanin Pigment in the Fossil Record