Purification of hyperexpressed Bacillus subtilis tRNATrp cloned in Escherichia coli

Abstract
To study the effects of evolutionary sequence changes on the molecular interactions of tRNA inside the cell, the Bacillus subtilis tRNA(Trp) gene has been cloned into Escherichia coli JM109 under the control of the lac promotor. Hyperexpression of the gene in minimal medium upon induction yielded 28% of total tRNA in the form of B. subtilis tRNA(Trp). The tRNA(Trp) gene product was purified by the use of a single Vydac C4 high-performance liquid...
Paper Details
Title
Purification of hyperexpressed Bacillus subtilis tRNATrp cloned in Escherichia coli
Published Date
Apr 1, 1993
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