Three-dimensional Structure of Satellite Tobacco Mosaic Virus at 2·9 Å Resolution

Published on May 1, 1993in Journal of Molecular Biology5.07
· DOI :10.1006/jmbi.1993.1289
Steven B. Larson13
Estimated H-index: 13
(UCR: University of California, Riverside),
Stanley Koszelak12
Estimated H-index: 12
(UCR: University of California, Riverside)
+ 3 AuthorsAlexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Abstract The crystal structure of satellite tobacco mosaic virus (STMV) has been solved by a combination of multiple isomorphous replacement and molecular replacement methods and refined at 2·9 A resolution to a conventional R -factor of 0·215. SMTV, a T = 1 icosahedral virus, is the smallest whose structure has been determined. The coat protein is an eight-stranded "Swiss roll" β-barrel with an amino-terminal strand that extends away from the β-barrel by more than 60 A. This strand is primarily responsible for quaternary interactions within the capsid. The most arresting feature of the virus structure is the intimate association of each capsid protein dimer with a Watson-Crick base-paired segment of RNA double helix on the interior of the virion. The icosahedral 2-fold axis of each dimer pair is coincident with that of the central base-pair of each helical RNA segment whose helical axis is along the edge of the icosahedron. The helical RNA segments are seven base-pairs in length with a stacked base at each 3′ end so that a total of 16 nucleotides is clearly visible. The character of the RNA helix is somewhat different than any of the canonical forms. Assuming full occupancy, then approximately 45% of the total RNA genome is present in the electron density map. The close association of capsid with highly structured nucleic acid suggests that assembly of STMV is likely to be a highly co-operative process involving both protein and RNA. The nucleic acid is distributed within the virion with a high degree of order. The capsid protein is a true double helical RNA binding protein and a number of prominent interactions between protein and RNA can be clearly seen.
  • References (0)
  • Citations (47)
Cited By47
#1James A. Geraets (Ebor: University of York)H-Index: 2
#2Eric C. Dykeman (Ebor: University of York)H-Index: 17
Last.Reidun Twarock (Ebor: University of York)H-Index: 22
view all 5 authors...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#2John Day (UCI: University of California, Irvine)H-Index: 10
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
view all 3 authors...
#1Yingying Zeng (Georgia Institute of Technology)H-Index: 2
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
Last.Stephen C. Harvey (Georgia Institute of Technology)H-Index: 45
view all 5 authors...
#1Stephen W. Lane (University of Leeds)H-Index: 2
#2Caitriona Dennis (University of Leeds)H-Index: 8
Last.Simon E V Phillips S E (University of Leeds)H-Index: 42
view all 7 authors...
#1Yuri G. Kuznetsov (UAH: University of Alabama in Huntsville)H-Index: 1
#2Jeffrey J. Dowell (UAH: University of Alabama in Huntsville)H-Index: 1
Last.Alexander McPherson (UAH: University of Alabama in Huntsville)H-Index: 2
view all 5 authors...
#1Siyang Sun (Purdue University)H-Index: 16
#2Venigalla B. Rao (CUA: The Catholic University of America)H-Index: 34
Last.Michael G. Rossmann (Purdue University)H-Index: 107
view all 3 authors...
#1Christopher Forrey (UMass: University of Massachusetts Amherst)H-Index: 3
#2M. Muthukumar (UMass: University of Massachusetts Amherst)H-Index: 58
View next paperDouble-helical RNA in satellite tobacco mosaic virus