Cell-free synthesis of GFP under high temperature conditions

Previously, we have developed a system for cell-free protein synthesis that can be operated at high temperatures using a lysate of Thermococcus kodakaraensis. Here, we tested cell-free synthesis of green fluorescent protein (GFP) using the T. kodakaraensis system. A thermostable GFP derivative (tGFP) was used as a reporter protein. By changing the codon usage of tGFP gene for T. kodakaraensis, production of tGFP was detectable in a temperature...