Precise protein solubility determination by Laser confocal differential interference contrast microscopy
Abstract
We describe a method for precisely measuring the solubility of proteins in aqueous solution using laser confocal differential interference contrast microscopy. The method is based on the in situ observation of single steps on a protein crystal surface which allows a fast and precise determination of solubility as a function of temperature. To demonstrate the effectiveness of this novel approach the solubility dependence on temperature of glucose...
Paper Details
Title
Precise protein solubility determination by Laser confocal differential interference contrast microscopy
Published Date
Jun 1, 2009
Journal
Volume
311
Issue
13
Pages
3479 - 3484
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