Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.

Volume: 79, Issue: 5, Pages: 1718 - 1729
Published: Mar 1, 2013
Abstract
Escherichia coli-mycobacterium shuttle vectors are important tools for gene expression and gene replacement in mycobacteria. However, most of the currently available vectors are limited in their use because of the lack of extended multiple cloning sites (MCSs) and convenience of appending an epitope tag(s) to the cloned open reading frames (ORFs). Here we report a new series of vectors that allow for the constitutive and regulatable expression...
Paper Details
Title
Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.
Published Date
Mar 1, 2013
Volume
79
Issue
5
Pages
1718 - 1729
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