Using Temperature To Crystallize Proteins: A Mini-Review

The aim of this overview of the work of Marseille Nanoscience Center is to provide biocrystallographs that intend to tackle crystallization with practical examples of the effect of temperature on protein phase separation and crystallization. Crystallization involves two separate processes: nucleation and growth, which are rarely completely unconnected. Here we give four concrete examples illustrating how temperature can be used to crystallize...