Structural aspects of conformational changes in ligand binding by antibody fragments

Conformational changes in immunoglobulin fragments on complexation were first noted in trigonal crystals (ammonium sulphate form) of the Meg light chain dimer in 1974 (Edmundson et al., 1974). Additional studies with Meg crystals demonstrated how induced fit mechanisms increase the diversity of ligands which can be bound by a single active site (Ely et al., 1978; Edmundson el al., 1984, 1987, 1989, 1993; Edmundson and Ely, 1985). The...