Mutagenesis of Alkalophilic Cellulosimicrobium sp. CKMX1 for Hyper-Production of Cellulase-Free Xylanase in Solid State Fermentation of Apple Pomace

Cellulosimicrobium sp. CKMX1 isolated from mushroom compost, produced extracellular endo-1,4-β-xylanase (EC 3.2.1.8) at 35 °C and pH 8.0. In this study the wild strain of Cellulosimicrobium sp. CKMX1 was improved for enhanced xylanase production by using physical mutagen i.e. ultraviolet rays and chemical mutagens i.e. ethidium bromide (0.1–2.0 mg/ml) and ethyl methanesulfonate (5–70 mg/ml). Mutant (E5) with hyper xylanase production was...