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Protection by Recombinant Newcastle Disease Viruses (NDV) Expressing the Glycoprotein (G) of Avian Metapneumovirus (aMPV) Subtype A or B against Challenge with Virulent NDV and aMPV

Published on Jan 1, 2013in World Journal of Vaccines
· DOI :10.4236/wjv.2013.34018
Qingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture),
Jason P. Roth3
Estimated H-index: 3
+ 3 AuthorsL. Zsak28
Estimated H-index: 28
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Abstract
Avian metapneumovirus (aMPV) and Newcastle disease virus (NDV) are threatening avian pathogens that can cause serious respiratory diseases in poultry worldwide. Vaccination, combined with strict biosecurity practices, has been the recommendation for controlling these diseases in the field. In the present study, we generated NDV LaSota vaccine strain-based recombinant viruses expressing the glycoprotein (G) of aMPV, subtype A or B, using reverse genetics technology. These recombinant viruses, rLS/aMPV-A G and rLS/aMPV-B G, were characterized in cell cultures and evaluated in turkeys as bivalent, next-generation vaccines. The results showed that these recombinant vaccine candi-dates were slightly attenuated in vivo, yet maintained similar growth dynamics, cytopathic effects, and virus titers in vitro when compared to the parental LaSota virus. The expression of the aMPV G protein in recombinant virus-infected cells was detected by immunofluorescence. Vaccination of turkeys with rLS/aMPV-A G or rLS/aMPV-B G conferred complete protection against velogenic NDV, CA02 strain challenge and partial protection against homologous patho-genic aMPV challenge. These results suggest that the LaSota recombinant virus is a safe and effective vaccine vector and expression of the G protein alone is not sufficient to provide full protection against aMPV-A or -B infections. Ex-pression of other aMPV-A or -B virus immunogenic protein(s) individually or in conjunction with the G protein may be necessary to induce stronger and more protective immunity against aMPV diseases.
  • References (36)
  • Citations (7)
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References36
Newest
#1Haixia Hu (JLU: Jilin University)H-Index: 3
#2Jason P. Roth (USDA: United States Department of Agriculture)H-Index: 3
Last.Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
view all 6 authors...
#1Qingzhong Yu (ARS: Agricultural Research Service)H-Index: 3
#2Carlos N. Estevez (ARS: Agricultural Research Service)H-Index: 2
Last.Laszlo Zsak (ARS: Agricultural Research Service)H-Index: 5
view all 5 authors...
#1Jorge Luis Chacón (USP: University of São Paulo)H-Index: 10
#2Matheus Y. Mizuma (USP: University of São Paulo)H-Index: 2
Last.Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 16
view all 8 authors...
#1Ra Mi Cha (UMN: University of Minnesota)H-Index: 6
#2Mahesh Khatri (UMN: University of Minnesota)H-Index: 14
Last.J. M. Sharma (UMN: University of Minnesota)H-Index: 40
view all 3 authors...
#1C. J. Naylor (University of Liverpool)H-Index: 17
#2C. Lupini (UNIBO: University of Bologna)H-Index: 3
Last.Paul A. Brown (University of Liverpool)H-Index: 4
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#1Dhanasekaran Govindarajan (Virginia–Maryland Regional College of Veterinary Medicine)H-Index: 7
#2A Shin-Hee Kim (Virginia–Maryland Regional College of Veterinary Medicine)H-Index: 1
Last.Siba K. Samal (Virginia–Maryland Regional College of Veterinary Medicine)H-Index: 35
view all 3 authors...
#1Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
#2Carlos Estevez (USDA: United States Department of Agriculture)H-Index: 12
Last.L. Zsak (USDA: United States Department of Agriculture)H-Index: 28
view all 5 authors...
Cited By7
Newest
#1Haixia Hu (SWU: Southwest University)H-Index: 3
#2Jason P. Roth (Merial)H-Index: 3
Last.Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
view all 4 authors...
#1Fatma Eldemery (AU: Auburn University)H-Index: 1
#2Yufeng Li (USDA: United States Department of Agriculture)H-Index: 1
Last.Haroldo Toro (AU: Auburn University)H-Index: 18
view all 5 authors...
#1Kiril M. Dimitrov (ARS: Agricultural Research Service)H-Index: 10
#2Claudio L. Afonso (ARS: Agricultural Research Service)H-Index: 44
Last.Patti J. Miller (ARS: Agricultural Research Service)H-Index: 23
view all 4 authors...
#1Zhenyu Zhang (NEAU: Northeast Agricultural University)H-Index: 6
#2Wei Zhao (USDA: United States Department of Agriculture)H-Index: 5
Last.Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
view all 6 authors...
#1Wei Zhao (USDA: United States Department of Agriculture)H-Index: 5
#2Zhenyu Zhang (NEAU: Northeast Agricultural University)H-Index: 6
Last.Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
view all 4 authors...
#1Wei Zhao (USDA: United States Department of Agriculture)H-Index: 5
#2Stephen J. Spatz (USDA: United States Department of Agriculture)H-Index: 12
Last.Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
view all 7 authors...
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