The Cryo-EM Structure of a Translation Initiation Complex from Escherichia coli
Abstract
The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation...
Paper Details
Title
The Cryo-EM Structure of a Translation Initiation Complex from Escherichia coli
Published Date
Jun 1, 2005
Journal
Volume
121
Issue
5
Pages
703 - 712
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