Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

Automation and miniaturization are key issues of high-throughput research projects in the post-genomic era. The implementation of robotics and parallelization has enabled researchers to process large numbers of protein targets for structural studies in a short time with reasonable cost efficiency. However, the cost of implementing the robotics and parallelization often prohibit their use in the traditional academic laboratory. Fortunately,...