Application of the automated interpretation of electron density maps to Bence-Jones protein Rhe.

Published on Jun 1, 1976in Journal of Molecular Biology5.067
· DOI :10.1016/0022-2836(76)90277-1
Jonathan Greer6
Estimated H-index: 6
(Columbia University)
The automated system for interpreting electron density maps of proteins has been applied to a newly calculated map of Bence-Jones protein Rhe. In order to test the methods and criteria incorporated in the program system, interpretation of Rhe was performed independently of the interpretation by Wang et al. (1974, 1975a,b†), who have used classical Richards box techniques. The automated system produced a single polypeptide chain which accounts for the whole molecule. Much of the secondary structure is detected and atomic co-ordinates are built for most of the main-chain atoms. The results indicate that the program system is able to interpret and build provisional main-chain co-ordinates for an electron density map of reasonable quality.
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