H-ras expression, genetic instability, and acquisition of metastatic ability by rat prostatic cancer cells following v-H-ras oncogene transfection

To study the relationship between metastatic ability, mutated H‐ ras expression, and genetic instability, a cloned, nonmetastatic rat prostatic cancer cell line (AT2.1) was transfected with the v‐H‐ ras oncogene. The parental AT2.1 clone, 4 control transfectants (Neo/Only), and 9 v‐H‐ ras transfectants (Neo/Ras) were characterized with regard to their H‐ ras content by using Southern, Northern, and Western blot analysis and their biological...