Challenges of glycoprotein analysis by microchip capillary gel electrophoresis
Abstract
Glycosylations severely influence a protein's biological and physicochemical properties. Five exemplary proteins with varying glycan moieties were chosen to establish molecular weight (MW) determination (sizing), quantitation, and sensitivity of detection for microchip capillary gel electrophoresis (MCGE). Although sizing showed increasing deviations from literature values (SDS-PAGE or MALDI-MS) with a concomitant higher degree of analyte...
Paper Details
Title
Challenges of glycoprotein analysis by microchip capillary gel electrophoresis
Published Date
Jul 14, 2015
Journal
Volume
36
Issue
15
Pages
1754 - 1758
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