Purification of glucose oxidase from complex fermentation medium using tandem chromatography

Volume: 858, Issue: 1-2, Pages: 151 - 158
Published: Oct 1, 2007
Abstract
A fast and efficient purification method for recombinant glucose oxidase (rGOx) for flask fermentation scale (up to 2 L) was designed for the purposes of characterization of rGOx mutants during directed protein evolution. The Aspergillus niger GOx was cloned into a pYES2-αMF-GOx construct and expressed extracellularly in yeast Saccharomyces cerevisiae. Hydrophobic interaction (HIC)/size exclusion (SEC)-tandem chromatographic system was designed...
Paper Details
Title
Purification of glucose oxidase from complex fermentation medium using tandem chromatography
Published Date
Oct 1, 2007
Volume
858
Issue
1-2
Pages
151 - 158
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