Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

Genome-wide analysis of Cas9-DNA interactions in mammalian cells shows widespread binding but low levels of cleavage. Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is poorly understood. Here we mapped genome-wide binding sites of a catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes loaded with single...