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Effects of endothelial nitric oxide synthase tagSNPs haplotypes on nitrite levels in black subjects.

Published on Jan 1, 2013in Nitric Oxide3.371
· DOI :10.1016/j.niox.2012.10.002
Ingrid F. Metzger15
Estimated H-index: 15
(USP: University of São Paulo),
Marcelo R. Luizon14
Estimated H-index: 14
(USP: University of São Paulo)
+ 2 AuthorsJose E. Tanus-Santos46
Estimated H-index: 46
(USP: University of São Paulo)
Abstract
Abstract Haplotypes formed by clinically relevant polymorphisms in the endothelial nitric oxide synthase ( eNOS ) gene have been associated with variations in endogenous nitric oxide (NO) formation in white and black subjects. We examined whether further genetic variation and haplotypes of the eNOS gene, represented by the rs3918188, rs743506 and rs7830 tagSNPs (polymorphisms that represent the information of neighboring SNPs in linkage disequilibrium) affect endogenous NO formation in 181 healthy black subjects. We measured whole blood nitrite concentration as a marker of endogenous NO formation for each subject. We found that the heterozygotes CA for the tagSNP rs7830 ( P  = 0.0278, OR = 0.4839, CI 95% 0.2567–0.9121), as well as the haplotype “C–G–A” ( P  = 0.0068), were more common in subjects with low circulating whole blood nitrite concentrations compared with subjects with high circulating whole blood nitrite concentrations, although the genotype finding is not significant after correction for multiple comparisons. These findings suggest that these tagSNPs of the eNOS gene and haplotypes are associated with low levels of nitric oxide production in blacks, and could be used as a marker of cardiovascular risk.
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#2Ingrid F. Metzger (USP: University of São Paulo)H-Index: 15
Last. Jose E. Tanus-Santos (USP: University of São Paulo)H-Index: 46
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To the Editor: Salvi et al1 reported that the single nucleotide polymorphism (SNP) rs3918226 in the promoter of the endothelial NO synthase (eNOS) gene was associated with hypertension. Although no functional studies show how this SNP affects eNOS, the authors identified a potential binding site for ETS transcription factors directly next to rs3918226, thus suggesting a potential modulation of eNOS expression.1 NO is mainly produced by eNOS in endothelial cells and is oxidized to nitrite, a usef...
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#1Ulrich Förstermann (University of Mainz)H-Index: 78
#2William C. Sessa (Yale University)H-Index: 104
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Glaucoma, an irreversible retinal deterioration which results in progressive visual field loss along with decreased contrast and color sensitivity, is a multifactorial optic neuropathy characterized by apoptotic cell death of the retinal ganglion cells (RGCs) in the optic disc or retinal nerve fiber [1,2]. Previous studies suggested that vascular dysregulation play an important role in the etiology of glaucoma [3,4]. Recently, activation of nitric oxide synthase (NOS), an enzyme associated with ...
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