The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins
Abstract
Bacterial over-expression of proteins is a powerful tool to obtain soluble protein amenable to biochemical, biophysical and/or structural characterization. However, it is well established that many recombinant proteins cannot be produced in a soluble form. Several theoretical and empirical methods to improve soluble production have been suggested, although there is to date no universally accepted protocol. This report describes, and...
Paper Details
Title
The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins
Published Date
Apr 1, 2008
Volume
58
Issue
2
Pages
210 - 221
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