Moderate heat stress reduces the pH component of the transthylakoid proton motive force in light-adapted, intact tobacco leaves

We measured the Δ Ψ and ΔpH components of the transthylakoid proton motive force ( pmf ) in light‐adapted, intact tobacco leaves in response to moderate heat. The Δ Ψ causes an electrochromic shift (ECS) in carotenoid absorbance spectra. The light–dark difference spectrum has a peak at 518 nm and the two components of the pmf were separated by following the ECS for 25 s after turning the light off. The ECS signal was deconvoluted by subtracting...