CALORIMETRIC INVESTIGATION OF RIBONUCLEASE THERMAL DENATURATION

Thermal denaturation of ribonuclease has been investigated by scanning microcalorimetric, spectrophotometric and polarimetric techniques. It is shown that the temperature changes of ribonuclease are of a complicated character and may be subdivided by the temperature region into two qualitatively different stages : ( a ) the predenaturational stage, at which neither abrupt changes in physico‐chemical properties nor heat absorption occur, but...