Possible involvement of tyrosine kinase activation on expression of inducible NO synthase in glial cells

To clarify the expression pathway of inducible nitric oxide synthase (iNOS) in the brain, we examined the effects of interferon-γ(IFN-γ) and lipopolysaccharide (LPS) on expression of iNOS in glial cells cultured from neonatal rats, compared to those in macrophage cell line RAW264.7. NOS activities (NO2- accumulation) and l 30-kDa iNOS protein were induced 24 hr after treatment with IFN-γ or LPS in both glial cells and RAW264.7 macrophages. These...