Nickel-dependent metalloenzymes

Published on Feb 1, 2014in Archives of Biochemistry and Biophysics3.559
· DOI :10.1016/
Jodi L. Boer6
Estimated H-index: 6
(MSU: Michigan State University),
Scott B. Mulrooney17
Estimated H-index: 17
(MSU: Michigan State University),
Robert P. Hausinger53
Estimated H-index: 53
(MSU: Michigan State University)
This review describes the functions, structures, and mechanisms of nine nickel-containing enzymes: glyoxalase I, acireductone dioxygenase, urease, superoxide dismutase, [NiFe]-hydrogenase, carbon monoxide dehydrogenase, acetyl-coenzyme A synthase/decarbonylase, methyl-coenzyme M reductase, and lactate racemase. These enzymes catalyze their various chemistries by using metallocenters of diverse structures, including mononuclear nickel, dinuclear nickel, nickel–iron heterodinuclear sites, more complex nickel-containing clusters, and nickel-tetrapyrroles. Selected other enzymes are active with nickel, but the physiological relevance of this metal specificity is unclear. Additional nickel-containing proteins of undefined function have been identified.
Figures & Tables
  • References (131)
  • Citations (112)
📖 Papers frequently viewed together
99 Citations
173 Citations
43 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Anuradha Balasubramanian (University of Madras)H-Index: 6
#2Vishnuprabu Durairajpandian (University of Madras)H-Index: 1
Last. Karthe Ponnuraj (University of Madras)H-Index: 13
view all 6 authors...
Urease is an enzyme that catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide, and is found in plants, microorganisms and invertebrates. Although plant and bacterial ureases are closely related at amino acid and at the structural level, the insecticidal activity is seen only in the plant ureases. In contrast, both plant and bacterial ureases exhibit antifungal activity. These two biological properties are independent of its ureolytic activity. However, till date the mechanism(s) ...
25 CitationsSource
#1Mark A. FarrugiaH-Index: 5
#2Lee Macomber (MSU: Michigan State University)H-Index: 10
Last. Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
view all 3 authors...
Metalloenzymes often require elaborate metallocenter assembly systems to create functional active sites. The medically important dinuclear nickel enzyme urease provides an excellent model for studying metallocenter assembly. Nickel is inserted into the urease active site in a GTP-dependent process with the assistance of UreD/UreH, UreE, UreF, and UreG. These accessory proteins orchestrate apoprotein activation by delivering the appropriate metal, facilitating protein conformational changes, and ...
56 CitationsSource
#1Joseph C. Polacco (MU: University of Missouri)H-Index: 35
#2Paulo Mazzafera (State University of Campinas)H-Index: 43
Last. Tiago Tezotto (ESALQ: Escola Superior de Agricultura Luiz de Queiroz)H-Index: 11
view all 3 authors...
a b s t r a c t We propose experimental strategies to expand our understanding of the role of Ni in plants, beyond the Ni-metallocenter of urease, still the only identified Ni-containing plant enzyme. While Ni has been con- sidered an essential mineral for plants there is a clear lack of knowledge of its involvement in metabolic steps except the urease-catalyzed conversion of urea to ammonia and bicarbonate. We argue that urease (and hence, Ni) plays an important role in optimal N-use efficiency...
66 CitationsSource
#1Caleb J. AllpressH-Index: 7
#2Katarzyna Grubel (USU: Utah State University)H-Index: 12
Last. Lisa M. Berreau (USU: Utah State University)H-Index: 24
view all 5 authors...
Mononuclear Fe(II) complexes ([(6-Ph2TPA)Fe(PhC(O)C(R)C(O)Ph)]X (3-X: R = OH, X = ClO4 or OTf; 4: R = H, X = ClO4)) supported by the 6-Ph2TPA chelate ligand (6-Ph2TPA = N,N-bis((6-phenyl-2-pyridyl)methyl)-N-(2-pyridylmethyl)amine) and containing a β-diketonate ligand bound via a six-membered chelate ring have been synthesized. The complexes have all been characterized by 1H NMR, UV–vis, and infrared spectroscopy and variably by elemental analysis, mass spectrometry, and X-ray crystallography. Tr...
35 CitationsSource
#1Ellen P. BroeringH-Index: 2
#2Phan T. TruongH-Index: 2
Last. Todd C. HarropH-Index: 20
view all 4 authors...
Nickel-containing superoxide dismutases (NiSODs) represent a novel approach to the detoxification of superoxide in biology and thus contribute to the biodiversity of mechanisms for the removal of reactive oxygen species (ROS). While Ni ions play critical roles in anaerobic microbial redox (hydrogenases and CO dehydrogenase/acetyl coenzyme A synthase), they have never been associated with oxygen metabolism. Several SODs have been characterized from numerous sources and are classified by their cat...
33 CitationsSource
#1Khadine A. HigginsH-Index: 11
#2Carolyn E. CarrH-Index: 2
Last. Michael J. MaroneyH-Index: 40
view all 3 authors...
Nickel is an essential metal for a number of bacterial species that have developed systems for acquiring, delivering, and incorporating the metal into target enzymes and controlling the levels of nickel in cells to prevent toxic effects. As with other transition metals, these trafficking systems must be able to distinguish between the desired metal and other transition metal ions with similar physical and chemical properties. Because there are few enzymes (targets) that require nickel for activi...
39 CitationsSource
#1Shi-Lu Chen (BIT: Beijing Institute of Technology)H-Index: 7
#2Margareta R. A. Blomberg (Stockholm University)H-Index: 46
Last. Per E. M. Siegbahn (Stockholm University)H-Index: 71
view all 3 authors...
Ni-containing methyl-coenzyme M reductase (MCR) is capable of catalyzing methane formation and has recently been observed to also be able to catalyze the reverse reaction, the anaerobic oxidation of methane. The forward reaction has been extensively studied theoretically before and was found to consist of two steps. The first step is rate-limiting and the second step was therefore treated at a lower level. For an accurate treatment of the reverse reaction, both steps have to be studied at the sa...
27 CitationsSource
#2Sasaki DaisukeH-Index: 11
Last. Kunio MikiH-Index: 42
view all 4 authors...
[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the Ni insertion, whereas the other four Hyp proteins (HypCDEF) are required for the biosynthesis, assembly and insertion of the Fe(CN)2CO group. Over the last decades, a large number of...
22 CitationsSource
#1Lee Macomber (MSU: Michigan State University)H-Index: 10
#2Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
Nickel has long been known to be an important human toxicant, including having the ability to form carcinomas, but until recently nickel was believed to be an issue only to microorganisms living in nickel-rich serpentine soils or areas contaminated by industrial pollution. This assumption was overturned by the discovery of a nickel defense system (RcnR/RcnA) found in microorganisms that live in a wide range of environmental niches, suggesting that nickel homeostasis is a general biological conce...
117 CitationsSource
#1Eric L. CarterH-Index: 5
#2Dale E. Tronrud (OSU: Oregon State University)H-Index: 7
Last. Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
view all 5 authors...
Helicobacter mustelae, a gastric pathogen of ferrets, synthesizes a distinct iron-dependent urease in addition to its archetypical nickel-containing enzyme. The iron-urease is oxygen-labile, with the inactive protein exhibiting a methemerythrin-like electronic spectrum. Significantly, incubation of the oxidized protein with dithionite under anaerobic conditions leads to restoration of activity and bleaching of the spectrum. Structural analysis of the oxidized species reveals a dinuclear iron met...
43 CitationsSource
Cited By112
#1Sarah E. N. Brazeau (BU: Boston University)H-Index: 1
#2Frances Pope (BU: Boston University)
Last. Linda H. Doerrer (BU: Boston University)H-Index: 22
view all 6 authors...
Abstract A series of heteroleptic mixed phosphine/alkoxide 3d complexes was designed to evaluate PPh3 as a protecting group, stabilizing the metal coordination sphere prior to O2 exposure but oxidatively dissociating in the presence of O2 to allow facile O2 reduction by low coordinate metal centers. Complexes of the form [(Ph3P)2M(OC4F9)2] (M= Fe (1) , Co (2) , Ni (3) , Zn (4) ) and [(Ph3P)2M(pinF)] (M= Co (8) , Ni (9) , Zn (10) were prepared, along with related complexes with non-reactive L-don...
#1Xueyuan Yan (CAS: Chinese Academy of Sciences)
#2Jinshuai Song (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Chunsen Li (CAS: Chinese Academy of Sciences)H-Index: 17
view all 4 authors...
Abstract Combined QM/MM calculations and MD simulations are utilized to investigate the detailed mechanisms of reactions catalyzed by wild-type nickel-dependent quercetin 2,4-dioxygenase (Ni-QueD) and its Glu76Asp and Glu76Gln mutants. The conserved nickel-ligating Glu76 residue in the deprotonated form is found to be essential for initiating the catalytic reaction by proton coupled electron transfer process. The generated protonated Glu76 promotes the subsequent reaction by regulating hydrogen-...
#1Syed Raza Shah (University of Nizwa)H-Index: 1
#2Zarbad Shah (Bacha Khan University)H-Index: 5
Last. Ahmed Al-Harrasi (University of Nizwa)H-Index: 24
view all 9 authors...
#1Lin Wang (UNC: University of North Carolina at Chapel Hill)
#1Lin WangH-Index: 5
view all 3 authors...
The ammonia-oxidizing obligate aerobic chemolithoautotrophic gammaproteobacterium, Nitrosococcus oceani, is omnipresent in the world’s oceans and as such important to the global nitrogen cycle. We generated and compared high quality draft genome sequences of N. oceani strains isolated from the Northeast (AFC27) and Southeast (AFC132) Pacific Ocean and the coastal waters near Barbados at the interface between the Caribbean Sea and the North Atlantic Ocean (C-27) with the recently published Draft ...
#1Wajiha Khan (Government College University)
#2Muhammad Athar Abbasi (Government College University)H-Index: 9
Last. Sung Y. Seo (UPR-RP: UPRRP College of Natural Sciences)
view all 10 authors...
#1Aimee L. Brauer (UB: University at Buffalo)H-Index: 18
#2Brian S. Learman (UB: University at Buffalo)H-Index: 3
Last. Chelsie E. Armbruster (UB: University at Buffalo)H-Index: 17
view all 3 authors...
Proteus mirabilis is a Gram-negative uropathogen and frequent cause of catheter-associated urinary tract infection (CAUTI). One important virulence factor is its urease enzyme, which requires nickel to be catalytically active. It is therefore hypothesized that nickel import is critical for P. mirabilis urease activity and pathogenesis during infection. P. mirabilis strain HI4320 encodes two putative nickel import systems, designated Nik and Ynt. By disrupting the substrate binding proteins from ...
#1Jessica Sobotta (TUM: Technische Universität München)H-Index: 2
#2Thomas Geisberger (TUM: Technische Universität München)H-Index: 1
Last. Claudia Huber (TUM: Technische Universität München)H-Index: 21
view all 7 authors...
Carbon fixation, in addition to the evolution of metabolism, is a main requirement for the evolution of life. Here, we report a one-pot carbon fixation of acetylene (C2H2) and carbon monoxide (CO) by aqueous nickel sulfide (NiS) under hydrothermal (>100 °C) conditions. A slurry of precipitated NiS converts acetylene and carbon monoxide into a set of C2–4-products that are surprisingly representative for C2–4-segments of all four central CO2-fixation cycles of the domains Bacteria and Archaea, wh...
#1Fernando Giovannetti Macedo (USP: University of São Paulo)H-Index: 3
#2Elcio Ferreira Santos (USP: University of São Paulo)H-Index: 8
Last. José Lavres (USP: University of São Paulo)H-Index: 10
view all 3 authors...
Abstract Nickel availability in the soil is altered mainly by pH, which can indirectly be changed by base saturation (BS). The objective was to evaluate the effect of consecutive plants growth on Ni availability and enzymatic activities in the rhizosphere. It was evaluated the interaction between BS ratios (50% and 70%) and Ni application rates (0, 0.1, 0.5, 1.0, and 10.0 mg dm−3 of soil) on the availability of Ni in the rhizosphere. Two agricultural crops were grown in succession (soybean and s...
1 CitationsSource