Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis

Significance DNA, the blueprint of the cell, is constantly damaged by chemicals and radiation. Because DNA damage may cause cell death or mutations that may lead to diseases such as cancer, cells are armed with an arsenal of several distinct mechanisms for repairing the many types of DNA damage that occur. DNA repair capacity (DRC) varies among individuals, and reduced DRC is associated with disease risk; however, the available DRC assays are...